Comparison of different extraction methods of curcumin

Of: Chen Yanhong, Qin Bo, Zhang Yuanyuan, Cheng Wei, Lu Gui source, Ye Zuguang

[Abstract] Objective Extraction of Curcumin on the 5 different methods were compared. Method to the extraction of curcumin content was obtained as the evaluation index rate cream, preferably curcumin extraction process. Results 80 ℃ soaking ethanol extract of curcumin the highest levels of income for the optimal extraction of curcumin. Conclusions The high extraction of curcumin, simple, stable and feasible.

[Keywords:] turmeric, curcumin, extraction method

Turmeric (Curcuma longa L.) plants derived from turmeric, dry ginger roots, mainly in China's Sichuan, Yunnan, Guangxi, Guangdong, Fujian and Taiwan. Turmeric and warm, spicy, bitter, with Poxue qi, through The role of pain, tingling commonly used in Xiong Xie, amenorrhea, Zheng Jia, rheumatism, shoulder and arm pain, swelling or flutter [1]. turmeric, including curcumin derivatives chemical composition (curcumins), terpenoids (Terpenoids) , sterols (sterols), Carbohydrates (Carbohydrates) and trace elements. which curcuminoids including curcumin (curcumin), to methoxy curcumin (demethoxy-curcumin) and two to methoxy turmeric Su (bisdemethoxycur-cumin) [2]. curcumin (C21H20O6) G for the alcohol-soluble hydrocarbon diphenyl compounds, not soluble in water, slightly soluble in ether and benzene, heated soluble in ethanol, ethylene glycol, soluble in glacial acetic acid and alkali solution. curcumin at high temperature, acid, alkali or poor light stability of the environment [3], the extraction temperature is not too high. Currently, the main extraction methods are methanol, ethanol and organic solvent extraction, alkaline thermal reference, enzymatic extraction method, such as field assisted extraction, this experiment raised alkaline heat, enzymatic extraction, ethanol extraction, ethanol soaking extraction, ethanol extraction of 5 percolation method, the extraction Curcumin was obtained from gypsum content and the inspection rate compared to provide a reference for the study of curcumin and basis.

An instrument and reagent

FZ102 micro mill plant samples (medical instrument factory of Beijing Yong light), DZKW-S-4 Electric heated water bath (Guangming Medical Instrument Factory, Beijing, Wing), DZF-6050 vacuum oven (Shanghai Science and Technology Co., Ltd. a constant) , AB135-S Electronic Analytical Balance (Mettler, Switzerland - TOLEDO), Agilent 1100 HPLC (Agilent Technologies).
Turmeric (share health medicine plant in Beijing, Sichuan and Chongqing), curcumin standard (Pharmaceutical and Biological Products, batch number 110823-200603), hemicellulase (hemicellulase, Sigma), other chemicals used were of pure, HPLC analysis of the reagents used for chromatography pure.

2 Methods and Results

2.1 Extraction

2.1.1 turmeric extract alkaline reflux [2]

Turmeric sifted through a 40 mesh sieve, take 50 g, add water, 1% sodium hydroxide solution with a pH value adjusted to 9.2, extracted in boiling water 3 times, add water, herbs were the original weight 10,8,6 times. Extraction time respectively 60,54,30 min.

2.1.2 turmeric extract digested alkaline reflux [4]

Turmeric sifted through a 40 mesh sieve, take 50 g, plus 6 times the amount of water in 90 ℃ water bath for 1 h, cooling, adding hemicellulase 1.75 mg, and add water to 10 times the amount of 1% hydrochloric acid solution with pH value adjusted to 4.5, hydrolysis in water bath at 50 ℃ 2 h, the enzyme concentration was 0.35 mg / mL, digested with 1% sodium hydroxide solution to adjust pH value of 9.2, according to "2.1.1" under way with the alkaline extraction.

2.1.3 turmeric ethanol extract

Turmeric sifted through a 40 mesh sieve, take 50 g, in 80% ethanol refluxing extract 3 times, 3 times the amount of ethanol were 6 times the weight of the original ingredients. Extraction time every 2 h.

2.1.4 ethanol soaking turmeric extract

Turmeric sifted through a 40 mesh sieve, take 50 g, in 80% ethanol heated to 80 ℃, heat soaking extracted 3 times and ethanol are 6 times the weight of the original ingredients. Extraction time every 2 h.

2.1.5 turmeric extract ethanol percolation [5]

Turmeric sifted through a 40 mesh sieve, to take 100 g, 80% ethanol after 6 h immersion dark percolation extraction and ethanol is 4 times the weight of the original herbs, glistening speed of 9 mL / (min · kg), collected 3.3 times the weight of glistening liquid medicine.

2.2 Determination of extract yield

After extraction by the combined extract, filtration, the filtrate after 60 ℃ water bath for solvent recovery vacuum concentrated to a certain concentration. Vacuum drying to constant weight, may extract, precision weighing, calculating the yield of extract [extract was rate (%) = extract weight (g) / amount of herbal medicine (g) × 100%], the results in Table 1.

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2.3 Determination of curcumin content

2.3.1 Chromatographic conditions

Column: Kromasil C18 (250 mm × 4.6 mm, 5 μm), pre-column: Kromasil C18 (7.5 mm × 4.6 mm, 5 μm), detection wavelength 430 nm, mobile phase: acetonitrile -4% acetic acid (48:52 ), column temperature 30 ℃, the flow rate 1.0 mL / min.

2.3.2 Preparation of standard curve

Curcumin accurately weighed amount of reference substance, add methanol dissolved and set the volume, the preparation of 11.84 μg / mL of the reference solution. 3,5,10,12,15,18 μL of each sample, the chromatographic conditions were determined by peak area. To the sample volume as abscissa and peak area for the vertical axis, the linear regression, the regression equation: Y = 4 827.3X-10.464, R2 = 0.999 5, r = 0.999 7, the results showed that curcumin at 0.035 52 ~ 0.213 12 μg sample between the peak area and the amount of linear relationship.

2.3.3 Determination of sample

Preparation of the reference substance into 9.944 μg / mL in methanol solution. Weigh the appropriate amount of sample extract powder, set 50 mL flask, diluted with methanol to the mark, and then set Precision Pipette 2 mL 10 mL flask, add methanol to volume, mix, filter membrane (0.45 μm), taken as a sample solution filtrate added. In the chromatographic conditions, standard, and samples were injection 10 μL, Jiang Huang Sufeng area measured by peak area of ​​the content of curcumin. The results in Table 2.
W [1 g of each extract containing curcumin (mg)] = (sample peak area / reference standard size) × 9.944 (μg / mL) ÷ sample concentration (mg / mL)

Curcumin content (mg) = W × extract weight (g)

Curcumin content (g/100 g herbs) = curcumin content (mg) × 10-3 / medicine amount (g) × 100%

2.4 Analysis

(See Table 1, Table 2) Table 1 Different Methods turmeric extract yield (Table 2 slightly different extracts of curcumin content (slightly Note: extract yield score = (30 / maximum extract yield) × extract yield, curcumin score = (70 / Max curcumin content) × curcumin content, integrated score = score + curcumin extract yield rate

From the above results can be obtained by ethanol extraction temperature and emulated the highest content of curcumin extracted, while the method is simple, stable and feasible.

3 Discussion

Modern pharmacological studies have shown that curcumin has anti-inflammatory, anti-virus, anti-fungal microbial, anti-tumor, anti-oxidation, scavenging free radicals, anti-aging, lowering blood pressure, protect the myocardium, inhibition of vascular remodeling and anti-atherosclerotic, anti-gall stones, protect liver and kidney, to protect the skin and anti-depressant and other pharmacological effects. Meanwhile, curcumin, or by the UN Food and Agriculture (FAO) and World Health Organization (WHO) approved the high safety of natural food coloring.

Although the use of turmeric, enzymatic hydrolysis can degrade the cell wall and the role of interstitial cells of cellulose, hemicellulose and other substances to improve the extraction rate of curcumin, but this experiment the effect of curcumin extracted enzyme is not obvious. Taking into account Turmeric heat resistance is poor, we took 80 ℃ soaking extraction method, to avoid degradation due to high temperature single curcumin, in order to obtain a higher extraction rate.

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