Treatment of malignant tumors rhino Pills Recent Clinical and Experimental Research


[Keywords:] rhino yellow pills, cancer, clinical research, experimental research, review


Huang Wan Qing Wang Hongxu rhino from book <<Surgical Treatment of the whole set of volume IV of Health>>, a West Pill, by bezoar, musk, frankincense, myrrh, yellow rice, smashed the pills for the treatment of breast rock, scrofula, sputum nuclear, flow injection, lung abscess, intestinal carbuncle embolism. are closing contained in <<The Ministry of Health standards for traditional Chinese medicine prescription pharmaceutical preparations>> ninth book, is the ideal anti-cancer clinical medicine . In recent years, many physicians on the basis of the CD in its time from side made a good clinical efficacy, and pharmacological effects on the growing number of experimental studies, the author is finishing below.


Clinical Research 1


1.1 Lung Cancer


Kuo et al [1] 60 patients with advanced non-small cell lung cancer (NSCLC) were randomly divided into treatment group and control group (30 cases), 2 groups herein are chemotherapy, the treatment group were treated with the West Pill, 3 g / time, 2 times / d. Results: The treatment group and control group clinical benefit rates were 46.67% and 40.00%, 1 year survival were 50.00% and 43.33%, difference between 2 groups was statistically significant (P <0.05 .) West Pill suggest more effective than chemotherapy alone in combination chemotherapy can improve survival of 1 year to reduce the side effects of chemotherapy. Shaw's [2] 60 patients with advanced lung cancer patients were divided into treatment group and control group (n = 30 ), are to receive 4 cycles of chemotherapy. treatment group West Capsule, 20 g / time, 3 times / d, 2 months for a course of treatment. Results: The treatment group and control group was 80.0% and 46.7 % difference between the 2 groups was statistically significant (P <0.05). treatment groups in clinical symptoms, the median survival and quality of life improve so much better than the control group (P <0.05). CONCLUSION: Chinese medicine, radiotherapy, Comprehensive treatment of lung cancer chemotherapy more effective than chemotherapy group, and patients with good compliance. Xu et al [3] treated 34 patients with chemotherapy failed Ⅲ B, Ⅳ of NSCLC patients, with traditional Chinese medicine treatment of hippocampal granule yellow rhinoceros (Rhinoceros Huang Wan plus hippocampus, and notoginseng), 4 capsules / time, 3 times / d, qd for 3 months. investigation of peripheral blood T cell subsets, CD4, CD4/CD8, NK cell activity increased, CD8 decreased compared with that before treatment There were significant differences (P <0.01), suggest enhanced immune function in patients, and improve the quality of life of patients can be as salvage therapy after failure of chemotherapy, especially for serious liver and kidney dysfunction and bone marrow function should not be inhibited to continue chemotherapy or advanced cachexia in patients with NSCLC unsuitable for chemotherapy use. Di s [4] reported that the West Yellow Tang Six Gentlemen Pill 2 cases of patients with primary lung cancer, oral West Pill, each 3 g, per day 2, with survival of more than 2 years.


1.2 liver


Chung's [5] Huang Wan Fang flavored with rhinoceros rhinoceros made of yellow capsule Compound 60 cases of liver cancer patients, 2 times a day 2 capsules, 2 months as a course of treatment, 153 treatment were observed, the results of partial remission 3 cases, 47 cases stable, deteriorated in 10 cases, no abnormal changes in liver and kidney function. Liang et al [6] treatment of primary liver cancer in 1 case, to use the line Stasis Decoction addition to disease, the other day morning and at night rhino Pill 1 pill to ginseng stew and soup with ginseng and Yang Rong, etc. with therapeutic, with survival past 6 years.


1.3 Breast Cancer


That's [7] rhino yellow pill treatment for breast cancer in 1 case, the first to clear the air with Xiaoyaosan Modified machine, breaking the accumulation of yellow pill after rhino Modified main with their breast cancer, plus monkey dates, notoginseng, Hill arrowhead such system for the pills, 2 pills a day, with the card during the addition and subtraction, breast lumps off. After 6 years after the follow-up, no bilateral breast lumps.


1.4 Other tumors


Down [8] treatment of patients with chronic lymphocytic leukemia in 1 case, rhino yellow pill daily 4.5 g, 2 ~ 3 times a day orally, together with Yiqiyangyin detoxification side, once every 2 months, anemia improved. Rhinoceros after the yellow used in conjunction Pill 31 months, his condition remained stable. Wu [9] treatment of esophageal squamous cell carcinoma in the lower part, side with the West Pill 6 g, 2 times a day, supplemented by raw Pinellia, raw bile Southern Star, ginger, and frequency of services Jiantang 10 months later, review the barium meal, no significant changes in the tumor. Tam [10] treatment of gallbladder cancer with liver metastasis West Pill, syndrome differentiation using traditional Chinese medicine Huang Wan Western Canada, daily 6 g, 1 year follow observed many times review showed a slight narrowing of tumor lesions. The other cases of bone metastasis after radiotherapy, chemotherapy and other anti-cancer medicine after the treatment had no significant effect, required analgesics. plus yellow pill with the West after the sharp drop in patients with pain. February months after the review of ECT, the former significantly reduced bone metastases, insist on taking the West Pill for 2 years. Down [11] The western yellow capsule combined with chemotherapy for cancer, 56 cases were randomly divided into treatment group (TCM + chemotherapy = 30) and control group (chemotherapy alone) in 26 cases. Capsule treatment group to the West, 8 pills each time, 2 times daily, 21 d as a course of treatment, once every 4 courses. the treatment group and control group was were 56.7% and 26.9%, the treatment group was the quality of life improvement than the control group (P <0.05).


Experimental Study 2


2.1 in vitro


2.1.1 The role of liver cancer cells Xiong et al [12-13] using MTT assay (MTT) test compound rhino Pills human hepatoma cells (Bel-7402) The inhibition rate of 39% results .10%, 15%, 20% of the group with serum inhibition of cell growth rate was higher and 2.5%, 5% group (P <0.05), serum concentration and inhibition rate showed a linear correlation. Tip Compound rhinoceros Pills on the growth of hepatoma cells in vitro to some extent. Another analysis by flow cytometry cell cycle distribution, display containing serum treatment 24 h, S phase cells increased, G2-M phase decreased, for 48 h,, G0 / 1 phase cells increased, S phase cells decreased (P <0.01). Tip rhinoceros Pills compound containing serum can interfere with cell cycle of human liver cancer cell lines. Xiong et al [14] with the rhino Yellow Pill Drug Serum effect on Bel-7402, reduced cell size observed in some nuclei break down, showing different sizes and irregular shape of the fluorescence staining of nuclear debris or plum-shaped type. chromatin condensation, and condensation into pieces, gathered in the membrane surrounding , crescent-shaped or kidney-shaped, and the number of apoptotic cells with the drug-containing serum concentration and action time and more.


Lee et al [15] with different concentrations of serum containing rhino Pills role different time Bel-7402 and found that the killing rate of up to 75%, significantly higher (P <0.01). Bel-detected by SASB 7402 Bcl-2, C-myc gene expression decreased, p53 protein expression increased. Lee et al [16] by flow cytometry rhino Pill (containing serum)-induced apoptosis of Bel-7402 was found , DNA agarose gel electrophoresis showed a clear DNA ladder, while the intracellular free calcium concentration was significantly higher in early apoptosis obvious induction of apoptosis may be one of the mechanisms.


Kim et al [17] found that extracts of the West Pill (100,200 μL / mL) significantly reduced the secretion of liver cancer cell SMMC7721 vascular endothelial growth factor (VEGF) levels (P <0.05, P <0.01) and the inhibition has a dose-dependent .5 d when its extract 200 μL / mL was significantly lower than the control group SMMC7721 secretion of pro-MMP-9, MMP-9, pro-matrix metalloproteinase-2 activity (P < 0.05, P <0.01), 7 d was more obvious, 2 concentrations were significantly lower activity (P <0.05, P <0.01), and there is a dose dependent manner. Kim [18] using flow cytometry determinations the concentration of 2 West Pill, SMMC7721 in the G0-G1 phase cells was significantly lower than the control group (P <0.01), 200 μL / mL the G2-M phase increased (P <0.01), that could specifically West Pill of the tumor cells to arrest in G2-M phase.


2.1.2 Liang on breast cancer cells [19] with the MTT assay 10%, 15%, 20% of the West, yellow pill containing serum group on human breast cancer cells (MCF-7) growth inhibition, were significantly higher than the negative control group (P <0.01) and 2.5% and 5% group, the serum concentration and inhibition rate showed a linear correlation. cycle analysis by flow cytometry showed that treatment with serum 24 h, S phase cells increased, G2 -M phase cells decreased after 48 h treatment, G0 / 1 phase cells increased, S phase cells decreased. Mingxi Huang Wan said the early days of serum containing cells into the G2-M phase, the cells arrest at the later stage G0 / 1 期, infer the mechanism is mainly manifested in the majority of cells arrest early in the DNA synthesis, leading to cancer cell apoptosis. Kim et al [20] were determined by MTT rhinoceros Pills Extracts on MDA-MB-231, SMMC7721, T24 , HL-60, A549 tumor cell proliferation, significantly inhibited (P <0.05, P <0.01), and a dose-dependent relationship to MDA-MB-231, SMMC7721 as sensitive. Conclusion: rhino Yellow Pill a variety of human tumor cells was significantly inhibited, but the sensitivity there is a big difference.


2.2 In vivo


Down et al [21] established mouse model of S180 solid tumor 60, were randomly divided into 4 groups, and let the negative control group. Results: The tumor inhibitory rate of 55.4% in treatment group was significantly higher than negative group (P <0.05), and higher than those in yellow pill with the West, or 5 - fluorouracil (5-Fu) group (P <0.05). prompted the West and 5-Fu Huang Wan synergistic effect. Kim et al [18] to give transplanted tumor U14 West Pill mouse model, cell cycle was determined by flow cytometry. Results: West Pill causes high dose group of U14 cells in G0-G1 cells was significantly lower than model group (P <0.01), G2-M phase cells increased (P <0.01). show West Pill tumor cells can be arrested at G2-M phase, and thus play inhibitory effect.


3 Conclusion


Huang Wan in the modern rhinoceros is widely used in clinical treatment of cancer, especially in the lung, liver, breast cancer treatment in cancer patients with chemotherapy can increase survival and improve the outcome. But only in vitro study learned The serum containing reached by interfering with cell cycle inhibitory role, mainly arrested cells entered the early G2-M phase, the cells arrest at the later stage G0 / 1 phase, so that the cell apoptosis. in vivo study, the main block cells in G2-M phase, but little research areas, and its mechanism has not been clear. In addition, different types of rhino yellow pill on specific aspects of cancer treatment remains to be further studied.


[References]


1 Guo Hui, Tian Fei, Gu Wenjuan, et al. West Pill combination chemotherapy in advanced non-small cell lung cancer [J]. Chinese medicine, 2008,17 (1) :22-23.


2 Xiao-lang, Li Qi, Yan Ping. West Capsule enhance the role of radiotherapy and chemotherapy lung cancer clinical observations [J]. Tianjin Pharmacy, 2007,19 (6) :34-36.


3 Xu Kai, Chenda Can, Li Liuning, et al. Hippocampal failure of chemotherapy treatment of rhino yellow particles Ⅲ B, Ⅳ non-small cell lung cancer [J]. Of traditional Chinese medicine, 2003,25 (5) :12-13.


4 Di above, Song Liqun. Song Liqun Experience in the treatment of primary lung cancer corner [J]. Liaoning Traditional Chinese Medicine, 2006,33 (9) :1190-1191.


5 Zhongwei Ling, Wang Xinhua. Compound the West Capsule Treatment of 60 cases of hepatocellular carcinoma [J]. Chinese folk medicine, 2003,11 (9) :56-57.


6 Liang is, Liang Jianbo. Name of the old Chinese Liang Jianbo Experience a treatment of hepatocellular carcinoma [J]. Practical Medicine, 1994,10 (2): 120.


7 It was Robinson. Rhinoceros yellow pill treatment for breast cancer 1 case [J]. Journal of Traditional Chinese Medicine, 1998,

5 (5): 39.

8 Tang by the ruler. The traditional Chinese medicine with traditional Chinese medicine treatment of cancer leukemia research [J]. Journal of Traditional Chinese Medicine, 1998,18 (10) :583-584.


9 Wu Mei. Rhinoceros Pills Examples of clinical application [J]. Chinese folk medicine, 2007,15 (11) :32-33.


10 Tan Ping. West Pill treatment of malignant application experience [J]. Journal of Traditional Chinese Medicine, 2001,11 (4): 224.


11 Tang Jingwen. Western yellow capsule combined with chemotherapy for cancer clinical research [J]. Of Traditional Chinese Medicine, 2008,23 (5) :59-60.


12 Xiong Ying, Kong Xiaoyun, Chen as a mountain, and so on. Rhinoceros Pills compound containing serum in vitro anticancer effect of [J]. Chinese Journal of Digestion, 2001,9 (4) :221-222.


13 Xiong Ying, Kong Xiaoyun, Chen like a mountain. Rhinoceros Pills compound containing serum on human liver cancer cell growth and cycle in rats [J]. Chinese Medical Science and Technology, 2001,8 (4) :217-218.


14 Xiong Ying, Kong Xiaoyun, Chen as a mountain, and so on. Rhinoceros Pill (containing serum) on human liver cancer cell apoptosis morphological study [J]. Journal of Clinical Gastroenterology, 2001,13 (2) :82-84 .


15 Li Lifang, Chen as a mountain, Liu Xinmin, et al. Rhinoceros Pill induced apoptosis of hepatoma cells and its mechanism [J]. Journal of Chinese Medicine, 2004,22 (1) :125-126.


16 Li Lifang, Chen as a mountain, Liu Xinmin. Rhinoceros Pills Bel-7402 cells induced apoptosis and changes in intracellular calcium concentration [J]. Clinical Hepatology, 2003,19 (6) :362-363.


17 Jin Shen Rui, Zhang Xinsheng, I wish Peter, et al. West Pill SMMC7721 hepatoma cells secreted vascular endothelial growth factor and matrix metalloproteinase 2,9 of [J]. Medicine, 2008,30 (7) :1079-1081 .


18 King Shen Rui, I wish Peter, Qinxu Hua, et al. West Pill SMMC7721 human hepatoma cells and mouse cycle of U14 cervical cancer cells [J]. MATERIA MEDICA, 2007,18 (11) :2782 -2783.


19 Liang Wenbo, Zhang Xuemei, Song Dan purpose. West Pill containing serum on the growth of human breast cancer cells [J]. MATERIA MEDICA, 2007,18 (6) :1371-1372.


20 gold, Shen Rui, I wish Peter, Qinxu Hua, et al. Rhinoceros yellow pill on various human cancer cell lines MDA-MB-231, SMMC7721, T24, HL-60, A549 proliferation [J]. Sichuan Traditional Chinese Medicine, 2006,24 ( 10) :10-12.


[21] Tang Xi, Ya Hu, Guo-qing. West Pill combined with fluorouracil anti-tumor effect [J]. Medicine, 2005,24 (9) :757-759.

Links to free paper download http://eng.hi138.com

[Keywords:] rhino yellow pills, cancer, clinical research, experimental research, review


CLC number: R273 Document code: D Article ID :1005 -5304 (2010) 04-0103-02



Huang Wan Qing Wang Hongxu rhino from book <<Surgical Treatment of the whole set of volume IV of Health>>, a West Pill, by bezoar, musk, frankincense, myrrh, yellow rice, smashed the pills for the treatment of breast rock, scrofula, sputum nuclear, flow injection, lung abscess, intestinal carbuncle embolism. are closing contained in <<The Ministry of Health standards for traditional Chinese medicine prescription pharmaceutical preparations>> ninth book, is the ideal anti-cancer clinical medicine . In recent years, many physicians on the basis of the CD in its time from side made a good clinical efficacy, and pharmacological effects on the growing number of experimental studies, the author is finishing below.


Clinical Research 1


1.1 Lung Cancer


Kuo et al [1] 60 patients with advanced non-small cell lung cancer (NSCLC) were randomly divided into treatment group and control group (30 cases), 2 groups herein are chemotherapy, the treatment group were treated with the West Pill, 3 g / time, 2 times / d. Results: The treatment group and control group clinical benefit rates were 46.67% and 40.00%, 1 year survival were 50.00% and 43.33%, difference between 2 groups was statistically significant (P <0.05 .) West Pill suggest more effective than chemotherapy alone in combination chemotherapy can improve survival of 1 year to reduce the side effects of chemotherapy. Shaw's [2] 60 patients with advanced lung cancer patients were divided into treatment group and control group (n = 30 ), are to receive 4 cycles of chemotherapy. treatment group West Capsule, 20 g / time, 3 times / d, 2 months for a course of treatment. Results: The treatment group and control group was 80.0% and 46.7 % difference between the 2 groups was statistically significant (P <0.05). treatment groups in clinical symptoms, the median survival and quality of life improvements, and significantly better than the control group (P <0.05). CONCLUSION: Chinese medicine, radiotherapy, Comprehensive treatment of lung cancer chemotherapy more effective than chemotherapy group, and patients with good compliance. Xu et al [3] treated 34 cases of failure of chemotherapy Ⅲ B, Ⅳ of NSCLC patients, with traditional Chinese medicine treatment of hippocampal granule yellow rhinoceros (Rhinoceros Huang Wan plus hippocampus, Panax, etc.), 4 capsules / time, 3 times / d, qd for 3 months. investigation of peripheral blood T cell subsets, CD4, CD4/CD8, NK cell activity increased, CD8 decreased compared with that before treatment There were significant differences (P <0.01), suggest enhanced immune function in patients, and improve the quality of life of patients can be as salvage therapy after failure of chemotherapy, especially for serious liver and kidney dysfunction and inhibition of bone marrow hematopoietic function should not be to continue chemotherapy or advanced cachexia in patients with NSCLC unsuitable for chemotherapy use. Di s [4] reported that the West Yellow Tang Six Gentlemen Pill 2 cases of patients with primary lung cancer, oral West Pill, each 3 g, per day 2, with survival of more than 2 years.


1.2 liver


Chung's [5] Huang Wan Fang flavored with rhinoceros rhinoceros made of compound Capsule in Treating 60 cases of liver cancer patients, 2 times a day 2 capsules, 2 months as a course of treatment, 153 treatment were observed, the results of partial remission 3 cases, 47 cases stable, deteriorated in 10 cases, no abnormal changes in liver and kidney function. Liang et al [6] treatment of primary liver cancer in 1 case, to use the line Stasis Decoction addition to disease, the other day morning and at night rhino Pill 1 pill to ginseng stew and soup with ginseng and Yang Rong, etc. with therapeutic, with survival past 6 years.


1.3 Breast Cancer


That's [7] rhino yellow pill treatment for breast cancer in 1 case, the first to clear the air with Xiaoyaosan Modified machine, breaking the accumulation of yellow pill after rhino Modified main with their breast cancer, plus monkey dates, Panax, mountain arrowhead such system for the pills, 2 pills a day, with the card during the addition and subtraction, breast lumps off. After 6 years after the follow-up, no bilateral breast lumps.


1.4 Other tumors


Down [8] treated one case of chronic lymphocytic leukemia patients, X Huang Wan daily 4.5 g, 2 ~ 3 times a day orally, together with Yiqiyangyin detoxification side, once every 2 months, anemia improved. Rhinoceros after the yellow used in conjunction Pill 31 months, his condition remained stable. Wu [9] treatment of esophageal squamous cell carcinoma in the lower, square yellow pill with the West, 6 g, 2 times a day, supplemented by raw Pinellia, raw bile Southern Star, ginger, and frequency of services Jiantang 10 months later, review the barium meal, no significant changes in the tumor. Tam [10] treatment of gallbladder cancer with liver metastasis West Pill, syndrome differentiation using traditional Chinese medicine Huang Wan Western Canada, daily 6 g, 1 year follow observed many times review showed a slight narrowing of tumor lesions. The other cases of bone metastasis after radiotherapy, chemotherapy and other anti-cancer medicine after the treatment had no significant effect, required analgesics. plus yellow pill with the West after the sharp drop in patients with pain. February months after the review of ECT, the former significantly reduced bone metastases, insist on taking the West Pill for 2 years. Down [11] The western yellow capsule combined with chemotherapy for cancer, 56 cases were randomly divided into treatment group (TCM + chemotherapy = 30) and control group (chemotherapy alone) in 26 cases. Capsule treatment group to the West, 8 pills each time, 2 times daily, 21 d as a course of treatment, once every 4 courses. the treatment group and control group was were 56.7% and 26.9%, the treatment group was the quality of life improvement than the control group (P <0.05).


Experimental Study 2


2.1 in vitro


2.1.1 The role of liver cancer cells Xiong et al [12-13] using MTT assay (MTT) test compound rhino Pills human hepatoma cells (Bel-7402) The inhibition rate of 39% results .10%, 15%, 20% of the group with serum inhibition of cell growth rate was higher and 2.5%, 5% group (P <0.05), serum concentration and inhibition rate showed a linear correlation. Tip Compound rhinoceros Pills on the growth of hepatoma cells in vitro to some extent. Another analysis by flow cytometry cell cycle distribution, display containing serum treatment 24 h, S phase cells increased, G2-M phase decreased, for 48 h,, G0 / 1 phase cells increased, S phase cells decreased (P <0.01). Tip rhinoceros Pills compound containing serum can interfere with cell cycle of human liver cancer cell lines. Xiong et al [14] with the rhino Yellow Pill Drug Serum effect on Bel-7402, reduced cell size observed in some nuclei break down, showing different sizes and irregular shape of the fluorescence staining of nuclear debris or plum-shaped type. chromatin condensation, and condensation into pieces, gathered in the membrane surrounding , crescent-shaped or kidney-shaped, and the number of apoptotic cells with the drug-containing serum concentration and action time and more.


Lee et al [15] with different concentrations of serum containing rhino Pills role different time Bel-7402 and found that the killing rate of up to 75%, significantly higher (P <0.01). Bel-detected by SASB 7402 Bcl-2, C-myc gene expression decreased, p53 protein expression increased. Lee et al [16] by flow cytometry rhino Pill (containing serum)-induced apoptosis of Bel-7402 was found , DNA agarose gel electrophoresis showed a clear DNA ladder, while the intracellular free calcium concentration was significantly higher in early apoptosis obvious induction of apoptosis may be one of the mechanisms.


Kim et al [17] found that extracts of the West Pill (100,200 μL / mL) significantly reduced the secretion of liver cancer cell SMMC7721 vascular endothelial growth factor (VEGF) levels (P <0.05, P <0.01) and the inhibition has a dose-dependent .5 d when its extract 200 μL / mL was significantly lower than the control group SMMC7721 secretion of pro-MMP-9, MMP-9, pro-matrix metalloproteinase-2 activity (P < 0.05, P <0.01), 7 d was more obvious, 2 concentrations were significantly lower activity (P <0.05, P <0.01), and there is a dose dependent manner. Kim et al [18] using flow cytometry determinations the concentration of 2 West Pill, SMMC7721 in the G0-G1 phase cells was significantly lower than the control group (P <0.01), 200 μL / mL the G2-M phase increased (P <0.01), that could specifically West Pill of the tumor cells to arrest in G2-M phase.


2.1.2 Liang on breast cancer cells [19] with the MTT assay 10%, 15%, 20% of the West, yellow pill containing serum group on human breast cancer cells (MCF-7) growth inhibition, were significantly higher than the negative control group (P <0.01) and 2.5% and 5% group, the serum concentration and inhibition rate showed a linear correlation. cycle analysis by flow cytometry showed that treatment with serum 24 h, S phase cells increased, G2 -M phase cells decreased after 48 h treatment, G0 / 1 phase cells increased, S phase cells decreased. Mingxi Huang Wan said the early days of serum containing cells into the G2-M phase, the cells arrest at the later stage G0 / 1 期, infer the mechanism is mainly manifested in the majority of cells arrest early in the DNA synthesis, leading to cancer cell apoptosis. Kim et al [20] were determined by MTT rhinoceros Pills Extracts on MDA-MB-231, SMMC7721, T24 , HL-60, A549 tumor cell proliferation, significantly inhibited (P <0.05, P <0.01), and a dose-dependent relationship to MDA-MB-231, SMMC7721 as sensitive. Conclusion: rhino Yellow Pill a variety of human tumor cells was significantly inhibited, but the sensitivity there is a big difference.


2.2 In vivo


Down et al [21] established mouse model of S180 solid tumor 60, were randomly divided into 4 groups, and let the negative control group. Results: The tumor inhibitory rate of 55.4% in treatment group was significantly higher than negative group (P <0.05), and higher than those in yellow pill with the West, or 5 - fluorouracil (5-Fu) group (P <0.05). prompted the West and 5-Fu Huang Wan synergistic effect. Kim et al [18] to give transplanted tumor U14 West Pill mouse model, cell cycle was determined by flow cytometry. Results: West Pill causes high dose group of U14 cells in G0-G1 cells was significantly lower than model group (P <0.01), G2-M phase cells increased (P <0.01). show West Pill tumor cells can be arrested at G2-M phase, and thus play inhibitory effect.


3 Conclusion


Huang Wan in the modern rhinoceros widely used in clinical treatment of cancer, especially in the lung, liver, breast cancer treatment in cancer patients with chemotherapy can increase survival and improve the outcome. But only in vitro study learned The serum containing reached by interfering with cell cycle inhibitory role, mainly arrested cells entered the early G2-M phase, the cells arrest at the later stage G0 / 1 phase, so that the cell apoptosis. in vivo study, the main block cells in G2-M phase, but little research areas, and its mechanism has not been clear. In addition, different types of rhino yellow pill on specific aspects of cancer treatment remains to be further studied.


[References]


1 Guo Hui, Tian Fei, Gu Wenjuan, et al. West Pill combination chemotherapy in advanced non-small cell lung cancer [J]. CHINESE MEDICINE, 2008,17 (1) :22-23.


2 Xiao-lang, Li Qi, Yan Ping. West Capsule enhance the role of radiotherapy and chemotherapy lung cancer clinical observations [J]. Tianjin Pharmacy, 2007,19 (6) :34-36.


3 Xu Kai, Chenda Can, Li Liuning, et al. Hippocampal failure of chemotherapy treatment of rhino yellow particles Ⅲ B, Ⅳ non-small cell lung cancer [J]. Of Traditional Chinese Medicine, 2003,25 (5) :12-13.


4 Di above, Song Liqun. Song Liqun Experience in the treatment of primary lung cancer corner [J]. Liaoning Traditional Chinese Medicine, 2006,33 (9) :1190-1191.


5 Zhongwei Ling, Wang Xinhua. Compound the West Capsule Treatment of 60 cases of hepatocellular carcinoma [J]. Chinese folk medicine, 2003,11 (9) :56-57.


6 Liang is, Liang Jianbo. Name of the old Chinese Liang Jianbo Experience a treatment of hepatocellular carcinoma [J]. Practical Medicine, 1994,10 (2): 120.


7 It was Robinson. Rhinoceros yellow pill treatment for breast cancer 1 case [J]. Journal of Traditional Chinese Medicine, 1998,

5 (5): 39.

8 Tang by the ruler. The traditional Chinese medicine with traditional Chinese medicine treatment of cancer leukemia research [J]. Journal of Traditional Chinese Medicine, 1998,18 (10) :583-584.


9 Wu Mei. Rhinoceros Pills Examples of clinical application [J]. Chinese folk medicine, 2007,15 (11) :32-33.


10 Tan Ping. West Pill treatment of malignant application experience [J]. Journal of Traditional Chinese Medicine, 2001,11 (4): 224.


11 Tang Jingwen. Western yellow capsule combined with chemotherapy for cancer clinical research [J]. Of Traditional Chinese Medicine, 2008,23 (5) :59-60.


12 Xiong Ying, Kong Xiaoyun, Chen as a mountain, and so on. Rhinoceros Pills compound containing serum in vitro anticancer effect of [J]. Chinese Journal of Digestion, 2001,9 (4) :221-222.


13 Xiong Ying, Kong Xiaoyun, Chen like a mountain. Rhinoceros Pills compound containing serum on human liver cancer cell growth and cycle in rats [J]. Chinese Medicine Technology, 2001,8 (4) :217-218.


14 Xiong Ying, Kong Xiaoyun, Chen as a mountain, and so on. Rhinoceros Pill (containing serum) on human liver cancer cell apoptosis morphological study [J]. Journal of Clinical Gastroenterology, 2001,13 (2) :82-84 .


15 Li Lifang, Chen as a mountain, Liu Xinmin, et al. Rhinoceros Pill induced apoptosis of hepatoma cells and its mechanism [J]. Journal of Chinese Medicine, 2004,22 (1) :125-126.


16 Li Lifang, Chen as a mountain, Liu Xinmin. Rhinoceros Pills Bel-7402 cells induced apoptosis and changes in intracellular calcium concentration [J]. Clinical Hepatology, 2003,19 (6) :362-363.


17 Jin Shen Rui, Zhang Xinsheng, I wish Peter, et al. West Pill SMMC7721 hepatoma cells secreted vascular endothelial growth factor and matrix metalloproteinase 2,9 of [J]. Medicine, 2008,30 (7) :1079-1081 .


18 King Shen Rui, I wish Peter, Qinxu Hua, et al. West Pill SMMC7721 human hepatoma cells and mouse cycle of U14 cervical cancer cells [J]. MATERIA MEDICA, 2007,18 (11) :2782 -2783.


19 Liang Wenbo, Zhang Xuemei, Song Dan purpose. West Pill containing serum on the growth of human breast cancer cells [J]. MATERIA MEDICA, 2007,18 (6) :1371-1372.


20 gold, Shen Rui, I wish Peter, Qinxu Hua, et al. Rhinoceros yellow pill on various human cancer cell lines MDA-MB-231, SMMC7721, T24, HL-60, A549 proliferation [J]. Sichuan Traditional Chinese Medicine, 2006,24 ( 10) :10-12.


[21] Tang Xi, Ya Hu, Guo-qing. West Pill combined with fluorouracil anti-tumor effect [J]. Medicine, 2005,24 (9) :757-759.

Links to free paper download http://eng.hi138.com

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